Volume 2, Issue 3-1, September 2017, Page: 32-36
In Vitro Culture of Turkish Origanum sipyleum L.
Başar Sevindik, Department of Horticulture, Faculty of Agriculture, University of Çukurova Balcalı, Adana, Turkey
Tolga İzgü, Department of Horticulture, Faculty of Agriculture, University of Ege Bornova, İzmir, Turkey
Özhan Şimşek, Department of Horticulture, Faculty of Agriculture, University of Çukurova Balcalı, Adana, Turkey
Mehmet Tütüncü, Department of Horticulture, Faculty of Agriculture, University of Ondokuz Mayıs Atakum, Samsun, Turkey
Pembe Çürük, Department of Horticulture, Faculty of Agriculture, University of Çukurova Balcalı, Adana, Turkey
Özer Yılmaz, Department of Biology, Faculty of Arts and Sciences, University of Uludağ, Nilüfer, Bursa, Turkey
Gönül Kaynak, Department of Biology, Faculty of Arts and Sciences, University of Uludağ, Nilüfer, Bursa, Turkey
Yıldız Aka Kaçar, Department of Horticulture, Faculty of Agriculture, University of Çukurova Balcalı, Adana, Turkey
Jaime A. Teixeira da Silva, Miki-cho Post Office, Ikenobe, Kagawa-ken, Japan
Yeşim Yalçın Mendi, Department of Horticulture, Faculty of Agriculture, University of Çukurova Balcalı, Adana, Turkey
Received: Apr. 20, 2017;       Accepted: Apr. 21, 2017;       Published: Jul. 17, 2017
DOI: 10.11648/j.ajpb.s.2017020301.16      View  1838      Downloads  121
Origanum sipyleum L. is a species that is endemic to Turkey with known medicinal and aromatic properties and with a long historical use in Turkish folk medicine. Micropropagation serves as one possible method to clone and protect O. sipyleum. In this study, nodes, which served as the explants, were cultured on Murashige and Skoog (MS) basal medium supplemented with different concentrations of BA (0.1, 0.5, 1.0 mgL-1) and GA3 (0.1, 0.2 mgL-1). Shoots were transferred to MS medium containing different concentrations of IBA (0, 0.5, 1, 1.5, 2, 2.5, 3 mgL-1) for root induction. Shoot regeneration, rooting, survival and acclimatization were possible. As many as 85% of nodes formed an average of 6 shoots/node on MS medium supplemented with 0.5 mgL-1 BA and 0.2 mgL-1 GA3. Rooting was most successful in 62.5% of shoots on MS medium with 1.5 or 2.5 mgL-1 IBA. Plantlets derived from all IBA combinations were transferred to autoclaved peat and perlite (1:1, v/v) and acclimatized in a greenhouse, with 79.1% survival. This protocol represents the first comprehensive in vitro regeneration protocol for endemic (Turkish) O. sipyleum.
BA, GA3, IBA, MS Medium, Tissue Culture
To cite this article
Başar Sevindik, Tolga İzgü, Özhan Şimşek, Mehmet Tütüncü, Pembe Çürük, Özer Yılmaz, Gönül Kaynak, Yıldız Aka Kaçar, Jaime A. Teixeira da Silva, Yeşim Yalçın Mendi, In Vitro Culture of Turkish Origanum sipyleum L., American Journal of Plant Biology. Special Issue: Plant Molecular Biology and Biotechnology. Vol. 2, No. 3-1, 2017, pp. 32-36. doi: 10.11648/j.ajpb.s.2017020301.16
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B. T. Ünal, A. Guvensen, A. E. Dereboylu, M. Öztürk, ''Variations in the proline and total protein contents in Origanum sipyleum L. from different altitudes of Spil mountain Turkey''. Pak. J. Bot., 45, 571-576, 2013.
M. Kürşat, I. Emre, O. Yilmaz, P. Erecevit, ''Antioxidant and antimicrobial activity in the seeds of Origanum vulgare L. subsp. gracile (C. Koch) Ietswaart and Origanum acutidens (Hand.-Mazz.) Ietswaart from Turkey'', Grasas y acites, 62 (4), 410-417, 2011.
T. Aşkun, G. Tümen, F. Satil, O. Yalçin, S. Modanlioğlu, ''Antimycobacterial activity some different Lamiaceae plant extracts containing flavonoids and other phenolic compounds,'' (In: Cardona PJ), editors Understanding Tuberculosis - New Approaches to Fighting Against Drug Resistance, InTech, Rijeka, Croatia, 309-336, 2012.
M. U. Yildirim, ''Micropropagation of Origanum acutidens (Hand.-Mazz.) Ietswaart using stem node explants,'' Scientific World J., 1-3, 2013.
M. Skoula, P. Gotsiou, G. Naxakis, C. B. Johnson, ''A chemosystematic investigation on the mono and sesquiterpenoids in the genus Origanum (Labiatae),'' Phytochemistry. 52 (4), 649-657, 1999.
S. Zein, S. Awada, S. Rachidi, A. Hajj, E. Krivoruschko, H. Kanaan, ''Chemical analysis of essential oil from Lebanese wild and cultivated Origanum syriacum l. (Lamiaceae) before and after flowering,'' J. Med. Plants Res., 5, 379-387, 2011.
K. H. C. Başer, T. Özek, M. Kürkçüoğlu, ''Composition of the essential oil of Origanum sipyleum L. of Turkish origin,'' J. Essential Oil Res., 4, 139-142, 1992.
M. Shahwan, T. Al-Qirim, A. Badder, '' Short-term feeding effect of Origanum syriacum crude extract on immobilization stress induced antioxidant defence changes in rat,'' J. Biol. Sci., 12 (7), 421-425, 2012.
P. H. Davis, ''Flora of Turkey and the East Aegean Islands (7)''. Edinburgh: Edinburgh University Press., 400-461, 1982.
A. E. Oluk and A. Çakir, ''Micropropagation of Origanum sipyleum L., an endemic medicinal herb of Turkey,'' African J. Biotechnol., 8 (21), 5769-5772, 2009.
T. Murashige, F. Skoog, ''A revised medium for rapid growth and bio assays with tobacco tissue cultures,'' Physiol. Plant., 15 (3), 473-497, 1962.
D. Özkum, ''In vitro shoot regeneration of oregano (Origanum minutiflorum O. Schwarz & Davis),'' Hacettepe J. Biol. Chem., 35 (2), 97-100, 2007.
O. L. Gamborg, R. Miller, K. Ojima, ''Nutrient requirements of suspension cultures of soybean root cells,'' Exp. Cell Res. 50 (1), 151-158, 1968.
G. N. Iconomou-Petrovich, I. Nianiou-Obeidat, ''Micropropagation of Origanum vulgare subsp. hirtum (Mt. Taygetos)''. In: Tsekos I, Moustakas M, (editors) Progress in Botanical Research, Springer, Berlin, 509-512, 1998.
D. Leelavathi and N. Kuppan, ''Callus induction and regeneration of multiple shoots from in vitro apical bud explant of Origanum vulgare, an important medicinal plant,'' Int. J. Res. Pharm. Chem., 898-903, 2013.
I. Morone-Fortunato, P. Avato, ''Plant development and synthesis of essential oils in micropropagated and mycorrhiza inoculated plants of Origanum vulgare L. ssp. hirtum,'' Plant Cell Tiss. Organ Cult., 93 (2), 139-149, 2008.
V. Lattanzio, A. Cardinali, C. Ruta, I. M. Fortunato, V. M. Lattanzi, V. Linsalata, N. Cicco, ''Relationship of secondary metabolism to growth in oregano (Origanum vulgare L.) shoot cultures under nutritional stress,'' Env. Exp. Bot., 65 (1), 54-62, 2009.
K. Ueno and K. Shetty, ''Prevention of hyperhydricity in oregano shoot cultures is sustained through multiple subcultures by selected polysaccharide-producing soil bacteria without re-inoculation,'' Applied Microbiol. Biotechnol., 50 (1), 119-124, 1998.
M. E. Goleniowski, C. Flamarique, P. Bima, ''Micropropagation of oregano (Origanum vulgare × applii) from meristem tips,'' In Vitro Cell. Dev. Biol. Plant. 39: 125-128, 2003.
O. Socorro, I. Tarrega, F. Rivas, ''Essential oils from wild and micropropagated plants of Origanum bastetanum,'' Phytochemistry, 48 (8), 1347-1349, 1998.
R. M. Arafeh, S. M. Mahmoud, A. R. Shibli, ''In vitro seed propagation of wild Syrian marjoram (Origanum syriacum),'' Adv. Hort. Sci., 17 (4), 241-244, 2003.
K. Shetty, O. F. Curtis, R. E. Levin, Witkowsky, W. Ang, ''Prevention of vitrification associated with in vitro shoot culture of oregano (Origanum vulgare) by Pseudomonas spp.,'' J. Plant Physiol., 147 (3), 447-451, 1995.
R. M. Arafeh, A. R. Shibli, M. Al-Mahmoud, M. A. Shatnawi, ''Callusing, cell suspension culture and secondary metabolites production in Persian oregano (Origanum vulgare L.) and Arabian oregano (O. syriacum L.),'' Jordan J. Agric. Sci., 2 (3), 274-281, 2006.
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