Volume 4, Issue 4, December 2019, Page: 75-83
Callus Culture for the Production of Therapeutic Compounds
Emmanuel Dabuwar Benjamin, Department of Pharmaceutical Microbiology and Biotechnology, University of Jos, Jos, Nigeria; Department of Biotechnology, Modibbo Adama University of Technology, Yola, Nigeria
Gali Adamu Ishaku, Department of Pharmaceutical Microbiology and Biotechnology, University of Jos, Jos, Nigeria
Fartisincha Andrew Peingurta, Department of Science Laboratory Technology, Modibbo Adama University of Technology, Yola, Nigeria
Abolade Samuel Afolabi, Biotechnology and Genetic Engineering Advanced Laboratory, Sheda Science and Technology Complex, Abuja, Nigeria
Received: Sep. 20, 2019;       Accepted: Oct. 8, 2019;       Published: Oct. 23, 2019
DOI: 10.11648/j.ajpb.20190404.14      View  26      Downloads  8
Plant-derived compounds retain a special place in the treatment of various diseases across the world. Their application cuts across every class of disease, where they are found to be often equal or of greater potency, safer and cheaper than so-called "orthodox" medicines. These advantages have led to great interest in the use of callus culture as a biotechnological tool for the harnessing of these useful therapeutic compounds. Callus culture techniques aim to increase the yield of active constituents in cultured plant cells and to produce novel products on a large scale. These techniques have been applied to produce various classes of therapeutic compounds from diverse plant species through empirical determination of ideal culture conditions and other methods. This review presents at a glance the recent advances being made in the field of callus culture for the production of therapeutic compounds, with the aim of showing that it is time for the full potentials of callus culture to be exploited on a scale that will prove a useful weapon in the arsenal of clinical therapeutics.
Callus Culture, Plant Growth Regulator, Elicitor, Precursor, Anticancer, Antiviral, Antioxidant, Therapeutic Nanoparticles
To cite this article
Emmanuel Dabuwar Benjamin, Gali Adamu Ishaku, Fartisincha Andrew Peingurta, Abolade Samuel Afolabi, Callus Culture for the Production of Therapeutic Compounds, American Journal of Plant Biology. Vol. 4, No. 4, 2019, pp. 75-83. doi: 10.11648/j.ajpb.20190404.14
Copyright © 2019 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
R. Eibl, “Plant cell culture technology in the cosmetics and food industries : current state and future trends,” pp. 8661–8675, 2018.
S. Ogita, “Plant cell, tissue and organ culture: The most flexible foundations for plant metabolic engineering applications,” Nat. Prod. Commun., vol. 10, no. 5, pp. 815–820, May 2015.
L. Yang, K. S. Wen, X. Ruan, Y. X. Zhao, F. Wei, and Q. Wang, “Response of plant secondary metabolites to environmental factors,” Molecules, vol. 23, no. 4. Multidisciplinary Digital Publishing Institute, p. 762, 27-Mar-2018.
N. Wisdom, E. Bassey, F. Jelani, G. Ishaku, U. Uwem, and S. Joseph, “Biochemical Studies of Ocimum sanctum and Olax subscorpioidea Leaf Extracts,” Br. J. Pharm. Res., vol. 12, no. 4, pp. 1–9, 2016.
T. Efferth, “Biotechnology Applications of Plant Callus Cultures,” Engineering, vol. 5, no. 1, pp. 50–59, 2019.
F. SKOOG and C. O. MILLER, “Chemical regulation of growth and organ formation in plant tissues cultured in vitro.,” Symp. Soc. Exp. Biol., vol. 11, pp. 118–30, 1957.
A. Sharma, A. K. Mathur, J. Ganpathy, B. Joshi, and P. Patel, “Effect of abiotic elicitation and pathway precursors feeding over terpenoid indole alkaloids production in multiple shoot and callus cultures of Catharanthus roseus,” Biologia (Bratisl)., vol. 74, no. 5, pp. 543–553, May 2019.
K. Kaur and P. K. Pati, “Stress-Induced Metabolite Production Utilizing Plant Hairy Roots,” in Hairy Roots, Singapore: Springer Singapore, 2018, pp. 123–145.
C. R. Singh, “Review on Problems and its Remedy in Plant Tissue Culture,” Asian J. Biol. Sci., vol. 11, no. 4, pp. 165–72, 2018.
S. Gonçalves and A. Romano, “Production of Plant Secondary Metabolites by Using Biotechnological Tools,” in Secondary Metabolites - Sources and Applications, InTech, 2018, pp. 81–99.
J. C. Cardoso, M. E. B. de Oliveira, and F. de C. Cardoso, “Advances and challenges on the in vitro production of secondary metabolites from medicinal plants,” Hortic. Bras., vol. 37, no. 2, pp. 124–132, Jun. 2019.
A. Amit Koparde, R. Chandrashekar Doijad, and C. Shripal Magdum, “Natural Products in Drug Discovery,” in Pharmacognosy-Medicinal Plants, IntechOpen, 2019.
S. Bhatia, “Plant Tissue Culture,” in Modern Applications of Plant Biotechnology in Pharmaceutical Sciences, Elsevier, 2015, pp. 31–107.
D. Pei, J. Xu, Q. Zhuang, H.-F. Tse, and M. a Esteban, “Induced pluripotent stem cell technology in regenerative medicine and biology.,” Adv. Biochem. Eng. Biotechnol., vol. 123, no. July 2015, pp. 127–141, 2010.
F. Bray, J. Ferlay, I. Soerjomataram, R. L. Siegel, L. A. Torre, and A. Jemal, “Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries,” CA. Cancer J. Clin., vol. 68, no. 6, pp. 394–424, Nov. 2018.
“Economic Impact of Cancer.” [Online]. Available: https://www.cancer.org/cancer/cancer-basics/economic-impact-of-cancer.html. [Accessed: 26-Aug-2019].
A. M. L. Seca and D. C. G. A. Pinto, “Plant secondary metabolites as anticancer agents: Successes in clinical trials and therapeutic application,” Int. J. Mol. Sci., vol. 19, no. 1, pp. 1–22, 2018.
M. Fridlender, Y. Kapulnik, and H. Koltai, “Plant derived substances with anti-cancer activity: From folklore to practice,” Frontiers in Plant Science, vol. 6, no. OCTOBER. Frontiers Media SA, p. 799, 2015.
L. Zhu and L. Chen, “Progress in research on paclitaxel and tumor immunotherapy.,” Cell. Mol. Biol. Lett., vol. 24, p. 40, 2019.
T. Zhou et al., “Transcriptome analyses provide insights into the expression pattern and sequence similarity of several taxol biosynthesis-related genes in three Taxus species,” BMC Plant Biol., vol. 19, no. 1, p. 33, Dec. 2019.
P. M. Naik and J. M. Al-Khayri, “Abiotic and Biotic Elicitors–Role in Secondary Metabolites Production through In Vitro Culture of Medicinal Plants,” in Abiotic and Biotic Stress in Plants - Recent Advances and Future Perspectives, IntechOpen, 2016.
M. Sarmadi, N. Karimi, J. Palazón, A. Ghassempour, and M. Hossein, “Improved effects of polyethylene glycol on the growth, antioxidative enzymes activity and taxanes production in a Taxus baccata L. callus culture,” Plant Cell, Tissue Organ Cult., vol. 0, no. 0, p. 0, 2019.
S. Yamamoto, S. Hayashi, S. Furusaki, and S. Shioya, “5-Aminolevulinic acid promotes callus growth and paclitaxel production in light-grown Taxus cuspidata suspension cultures,” Eng. Life Sci., pp. 1–32, 2014.
S. Wang et al., “Effect of elicitors, precursors and metabolic inhibitors on paclitaxel production by Taxus cuspidata cell culture,” J. For. Res., vol. 27, no. 6, pp. 1257–1263, Dec. 2016.
C. K. Jain, “Topoisomerases ☆,” in Reference Module in Life Sciences, Elsevier, 2017.
V. V. Raveendran, “Camptothecin-Discovery, Clinical Perspectives and Biotechnology,” vol. 3, no. 3, 2015.
K. Sakato, H. Tanaka, N. Mukai, and M. Misawa, “Isolation and Identification of Camptothecin from Cells of Camptotheca acuminata Suspension Cultures,” Agric. Biol. Chem., vol. 38, no. 1, pp. 217–218, Jan. 1974.
C. Veeresham and M. L. Shuler, “Camptothecin from callus cultures of Nothapodytes foetida,” Biotechnol. Lett., vol. 22, pp. 129–132, 2000.
T. Isah, “Production of camptothecin in the elicited callus cultures of Nothapodytes nimmoniana (J. Graham) Mabberly,” Chem. Pap., vol. 71, no. 6, pp. 1091–1106, 2016.
J. J. Krishnan, A. Gangaprasad, and K. Satheeshkumar, “Biosynthesis of Camptothecin from Callus and Cell Suspension Cultures of Ophiorrhiza mungos L. var. angustifolia ( Thw.) Hook. f.,” Proc. Natl. Acad. Sci. India Sect. B Biol. Sci., 2018.
H. N. Thriveni, G. Ravikanth, R. Vasudeva, K. N. Ganeshaiah, and R. Uma Shaanker, “Camptothecine and methoxy camptothecine from callus cultures of Miquelia dentata Bedd - A rare plant of the Western Ghats of India,” Indian J. Biotechnol., vol. 14, no. 1, pp. 123–126, 2015.
E. Martino et al., “Vinca alkaloids and analogues as anti-cancer agents: Looking back, peering ahead,” Bioorganic and Medicinal Chemistry Letters, vol. 28, no. 17. pp. 2816–2826, Sep-2018.
H. Barrales-Cureño, “Pharmacological applications and in vitro biotechnological production of anticancer alkaloids of Catharanthus roseus,” Biotecnol. Apl., vol. 32, no. 1, pp. 1101–1110, 2015.
K. K. Jagjit Kaur, Apoorva Singh, Teena Pathak, “Role of PGRs in Anticancer Alkaloids (Vincristine and Vinblastine) Production,” in Catharanthus Roseus: Current Research and Future Prospects, M. N. et Al., Ed. Springer International Publishing, 2017, pp. 309–319.
E. M. A. Al-Zuhairi and A. A. Obaid, “Effect of abscisic acid (aba) on the production of some secondary metabolites from callus of catharanthus roseus l. g. don in vitro,” ANBAR J. Agric. Sci., vol. 15, no. 1, pp. 318–326, 2017.
P. Pliankong, P. Suksa-Ard, and S. Wannakrairoj, “Chitosan Elicitation for Enhancing of Vincristine and Vinblastine Accumulation in Cell Culture of Catharanthus roseus (L.) G. Don,” J. Agric. Sci., vol. 10, no. 12, p. 287, 2018.
M. Maqsood and M. Abdul, “Yeast extract elicitation increases vinblastine and vincristine yield in protoplast derived tissues and plantlets in Catharanthus roseus,” Rev. Bras. Farmacogn., vol. 27, no. 5, pp. 549–556, Sep. 2017.
H. Pandey, P. Pandey, and S. Singh, “Production of anti-cancer triterpene ( betulinic acid) from callus cultures of different Ocimum species and its elicitation,” pp. 647–655, 2015.
M. K. Tripathi, N. Mishra, S. Tiwari, C. Shyam, S. Singh, and A. Ahuja, “Plant Tissue Culture Technology: Sustainable Option for Mining High Value Pharmaceutical Compounds,” Int. J. Curr. Microbiol. Appl. Sci., vol. 8, no. 02, pp. 1002–1010, 2019.
T. Jan, R. Qadri, B. Naqvi, A. Adhikari, S. Nadeem, and A. Muhammad, “A novel Salvialactomine from the callus culture of Salvia santolinifolia Boiss,” Nat. Prod. Res., vol. 32, no. 7, pp. 749–754, 2018.
M. G. Moloney, “Natural Products as a Source for Novel Antibiotics,” Trends Pharmacol. Sci., vol. 37, no. 8, pp. 689–701, Aug. 2016.
P. D. Gupta and T. J. Birdi, “Development of botanicals to combat antibiotic resistance,” J. Ayurveda Integr. Med., vol. 8, no. 4, pp. 266–275, 2017.
C. Cultures et al., “Investigating the Antimicrobial Potential of in- vitro Grown,” vol. 12, no. 1, pp. 43–48, 2019.
I. Chóez-guaranda et al., “Identification of lupeol produced by Vernonanthura patens ( Kunth) H. Rob. leaf callus culture Identification of lupeol produced by Vernonanthura,” Nat. Prod. Res., vol. 0, no. 0, pp. 1–5, 2019.
R. Rameshkumar et al., “Production of squalene with promising antioxidant properties in callus cultures of Nilgirianthus ciliatus,” Ind. Crops Prod., vol. 126, no. October, pp. 357–367, 2018.
A. Adebiyi, E. Bassey, R. Ayo, I. Bello, J. Habila, and G. Ishaku, “Anti-mycobacterial, Antimicrobial and Phytochemical Evaluation of Pulicaria crispa and Scoparia dulcis Plant Extracts,” J. Adv. Med. Pharm. Sci., vol. 7, no. 4, pp. 1–11, 2016.
F. Bongomin, S. Gago, R. O. Oladele, and D. W. Denning, “Global and multi-national prevalence of fungal diseases—estimate precision,” J. Fungi, vol. 3, no. 4, 2017.
N. P. Wiederhold, “Antifungal resistance: current trends and future strategies to combat,” Infect. Drug Resist., vol. 10, pp. 249–259, 2017.
A. Fausto, M. L. Rodrigues, and C. Coelho, “The still underestimated problem of fungal diseases worldwide,” Front. Microbiol., vol. 10, no. FEB, pp. 1–5, 2019.
R. Prasad, A. H. Shah, and M. K. Rawal, “Antifungals: Mechanism of Action and Drug Resistance,” in Yeast Membrane Transport, no. January, J. R. et Al., Ed. Springer International Publishing Switzerland, 2016, pp. 327–349.
L. Scorzoni et al., “Antifungal therapy: New advances in the understanding and treatment of mycosis,” Front. Microbiol., vol. 8, no. JAN, pp. 1–23, 2017.
D. Mathias, S. Hammantola, and G. Ishaku, “Isolation and Characterization of Bioflocculant-Producing Bacteria from Wastewater at Jimeta, Adamawa State,” J. Adv. Biol. Biotechnol., vol. 15, no. 1, pp. 1–7, 2017.
F. D. D. Auria et al., “Xanthones from roots, hairy roots and cell suspension cultures of selected Hypericum species and their antifungal activity against Candida albicans,” no. November, 2015.
S. Begum and S. Mirza, “Phytochemical Analysis and Antimicrobial Activity of Tissues and Callus Culture Extracts of Cichorium Intybus.,” Transylvanian Rev., vol. 26, no. 24, pp. 209–211, 2018.
G. A. Ishaku, A. A. Arabo, and E. E. Bassey, “Physicochemical Characterization and Antibacterial Activity of Senna occidentalis Linn,” vol. 6, no. January, pp. 9–18, 2016.
Q. Shen et al., “The Genome of Artemisia annua Provides Insight into the Evolution of Asteraceae Family and Artemisinin Biosynthesis,” Mol. Plant, vol. 11, no. 6, pp. 776–788, 2018.
F. Yuliani, W. S. Dewi, A. Yunus, and U. Siswanto, “The Study of Artemisinin Content in Callus Artemisia annua L. Cultures Elicited with Endophytic Fungi Aspergillus sp.,” Molekul, vol. 13, no. 2, p. 155, 2018.
M. A. El-Nabarawy, S. H. El-Kafafi, M. A. Hamza, and M. A. Omar, “The effect of some factors on stimulating the growth and production of active substances in Zingiber officinale callus cultures,” Ann. Agric. Sci., vol. 60, no. 1, pp. 1–9, 2015.
B. Ghasemi, R. Hosseini, and F. Dehghan Nayeri, “Effects of cobalt nanoparticles on artemisinin production and gene expression in Artemisia annua,” Turk. J. Botany, vol. 39, no. 5, pp. 769–777, 2015.
W. K. Kayani, B. H. Kiani, E. Dilshad, and B. Mirza, “Biotechnological approaches for artemisinin production in Artemisia,” World J. Microbiol. Biotechnol., vol. 34, no. 4, p. 0, 2018.
S. M. Tahir, I. S. Usman, M. D. Katung, and M. F. Ishiyaku, “The effect of plant growth regulators on callus initiation in wormwood (Artemisia annua L.),” Bayero J. Pure Appl. Sci., vol. 9, no. 1, p. 160, 2016.
A. Zebarjadi, S. Dianatkhah, P. Pour Mohammadi, and A. Qaderi, “Influence of abiotic elicitors on improvement production of artemisinin in cell culture of Artemisia annua L.,” Cell. Mol. Biol. (Noisy-le-grand)., vol. 64, no. 9, pp. 1–5, Jun. 2018.
A. J. et al., “Quinine, an old anti-malarial drug in a modern world: Role in the treatment of malaria,” Malar. J., vol. 10, pp. 1–12, 2011.
D. R. Pratiwi, Sumaryono, P. T. Sari, and D. Ratnadewi, “Cinchona cells performance in in vitro culture: Quinine alkaloid production with application of different elicitors,” IOP Conf. Ser. Earth Environ. Sci., vol. 185, no. 1, 2018.
G. Ishaku, “Nutritional Composition of Tamarindus indica fruit pulp,” J. Chem. Chem. Sci., vol. 6, no. 8, pp. 695–699, 2016.
V. Malayaman, G. B. M, and A. B. Kolar, “An efficient callus induction from Phyllanthus debilis Klein Ex Willd- a wild medicinal plant of Eastern Ghats, India An Efficient Callus Induction from Phyllanthus debilis Klein Ex Willd- A Wild Medicinal Plant of Eastern Ghats, India,” no. November, 2014.
M. Younas, S. Drouet, M. Nadeem, N. Giglioli-guivarc, C. Hano, and B. Haider, “Differential accumulation of silymarin induced by exposure of Silybum marianum L. callus cultures to several spectres of monochromatic lights,” J. Photochem. Photobiol. B Biol., p. #pagerange#, 2018.
M. L. Dos Santos, W. Quintilio, T. M. Manieri, L. R. Tsuruta, and A. M. Moro, “Advances and challenges in therapeutic monoclonal antibodies drug development,” Brazilian J. Pharm. Sci., vol. 54, no. Special Issue, pp. 1–15, 2018.
A. Malik, “Plantibodies: A New Approach For Immunomodulation in Human Health,” Biomed. J. Sci. Tech. Res., vol. 11, no. 2, pp. 8339–8340, 2018.
F. Southey, “Plant-based expression system produces higher yields than mammalian alternatives, says CEO,” 2018. [Online]. Available: https://www.biopharma-reporter.com/Article/2018/10/11/Plant-based-expression-system-produces-higher-yields-than-mammalian-alternatives-says-CEO#. [Accessed: 12-Sep-2019].
D. Jacomini et al., “Lipid profile and antiproliferative activity of callus cultures of Cereus peruvianus Mill,” Ind. Crops Prod., vol. 69, pp. 408–414, 2015.
S. Mohammed, A. A. Samad, and Z. Rahmat, “Agrobacterium-Mediated Transformation of Rice: Constraints and Possible Solutions,” Rice Sci., vol. 26, no. 3, pp. 133–146, 2019.
D. O. Govea-Alonso et al., “Assessment of Carrot Callus as Biofactories of an Atherosclerosis Oral Vaccine Prototype,” Mol. Biotechnol., vol. 59, no. 11–12, pp. 482–489, 2017.
J. Hidalgo, D., Abdoli -Nasab, M., Jalali-Javaran, M., Bru-Martínez, R., Cusidó, R. M., Corchete, P., & Palazon, “Biotechnological production of recombinant tissue plasminogen activator protein (reteplase) from transplastomic tobacco cell cultures.,” Plant Physiol. Biochem., vol. 118, pp. 130–137, 2017.
R. Kotcherlakota, S. Das, and C. R. Patra, Therapeutic applications of green-synthesized silver nanoparticles. Elsevier Inc., 2019.
N. Ochekpe, P. Olorunfemi, and N. Ngwuluka, “Nanotechnology and Drug Delivery Part 1: Background and Applications,” Trop. J. Pharm. Res., vol. 8, no. 3, pp. 265–274, 2009.
V. Reddy, N. Venkata, S. Kotakadi, and L. Domdi, “Biogenic silver nanoparticles : efficient and effective antifungal agents,” Appl. Nanosci., vol. 6, no. 4, pp. 475–484, 2016.
A. I. Journal et al., “Green synthesis of silver nanoparticles using transgenic Nicotiana tabacum callus culture expressing silicatein gene from marine sponge Latrunculia oparinae,” Artif. Cells, Nanomedicine, Biotechnol., vol. 46, no. 8, pp. 1646–1658, 2018.
R. I. Iyer and T. Panda, “Biosynthesis of Gold and Silver Nanoparticles Using Extracts of Callus Cultures of Pumpkin ( Cucurbita maxima),” J. Nanosci. Nanotechnol., vol. 18, no. 8, pp. 5341–5353, 2018.
I. Liguori et al., “Clinical Interventions in Aging Dovepress Oxidative stress, aging, and diseases,” Clin. Interv. Aging, vol. 13, pp. 757–772, 2018.
“Natural Antioxidants Market Size, Share |Industry Growth Report, 2022,” 2016. [Online]. Available: https://www.grandviewresearch.com/industry-analysis/natural-antioxidants-market. [Accessed: 12-Sep-2019].
F. P. Andrew and P. A. Ajibade, “Metal complexes of alkyl-aryl dithiocarbamates: Structural studies, anticancer potentials and applications as precursors for semiconductor nanocrystals,” J. Mol. Struct., vol. 1155, no. 2018, pp. 843–855, 2018.
A. Sarkar and G. Uma, “Natural Antioxidants-The Key to Safe and Sustainable Life,” Int. J. Latest Trends Eng. Technol., vol. 6, no. 3, pp. 460–466, 2016.
A. S. Hosny, F. M. Sabbah, and Z. E. El-bazza, “Studies on the microbial decontamination of Egyptian bee pollen by γ radiation,” Egypt Pharm. J, vol. 17, pp. 32–39, 2018.
N. Ahmad, N. Ahmad, and A. Rab, “Light-induced biochemical variations in secondary metabolite production and antioxidant activity in callus cultures of Stevia rebaudiana (Bert),” J. Photochem. Photobiol. B Biol., vol. 154, pp. 51–56, 2016.
B. Janarthanam, M. Gopalakrishnan, and T. Sekar, “Secondary Metabolite Production in Callus Cultures of Stevia rebaudiana Bertoni Secondary Metabolite Production in Callus Cultures of Stevia rebaudiana Bertoni,” no. November 2010, 2016.
Y. Chisti, “Strategies in Downstream Processing,” Biosep. Bioprocess., no. April 2008, pp. 2–30, 2008.
“Growing at a slower pace, world population is expected to reach 9.7 billion in 2050 and could peak at nearly 11 billion around 2100 | UN DESA | United Nations Department of Economic and Social Affairs,” 2019. [Online]. Available: https://www.un.org/development/desa/en/news/population/world-population-prospects-2019.html. [Accessed: 12-Sep-2019]. org.
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