Volume 3, Issue 1, March 2018, Page: 12-16
In Vitro Clonal Propagation of Musa Sp. Cultivar Gopi: A Palatable Banana of Tripura, India
Rabindra Kumar Sinha, Cytogenetics and Plant Biotechnology Lab., Department of Botany, Tripura University, Suryamaninagar, India
Puja Rani Saha, Cytogenetics and Plant Biotechnology Lab., Department of Botany, Tripura University, Suryamaninagar, India
Anath Bandhu Das, Department of Botany, Utkal University, Bhubaneswar, India
Satya Narayan Jena, Genetics and Molecular Biology, Council of Scientific and Industrial Research-National Botanical Research Institute, Lucknow, India
Sangram Sinha, Cytogenetics and Plant Biotechnology Lab., Department of Botany, Tripura University, Suryamaninagar, India
Received: Apr. 18, 2018;       Accepted: May 3, 2018;       Published: May 30, 2018
DOI: 10.11648/j.ajpb.20180301.13      View  831      Downloads  64
The present investigation has been concentrated on the establishment of an efficacious method for in vitro regeneration of a Musa cultivar Gopi of Tripura. Focus has been greatly anchored to the sterilization procedure for assuring successful aseptic culture establishment of this cultivar. The culture protocol for shoot induction and proliferation comprised of four different treatments in basal MS medium fortified with 6-Benzylaminopurine of varying concentrations. The optimum response was encountered in the treatment of BAP in MS medium at concentration of 8mg l-1 for shoot bud induction and multiplication. Subsequent shoot growth and multiplication was achieved through repeated subcultures in media containing lower concentration (4 mg l-1). Root initiation was stimulated and brought about by treatments with two different concentrations of Indole butyric acid. The best rooting was manifested in treatment with IBA at concentration of 2mg l-1. The present study is an approach towards successful establishment of a simple and rapid clonal propagation of a potent Musa cultivar Gopi having traditional and commercial value from this region of Tripura, North-east India.
In Vitro Regeneration, Musa Cultivar Gopi, Benzylaminopurine, Indole Butyric Acid, Clonal Propagation, Tripura
To cite this article
Rabindra Kumar Sinha, Puja Rani Saha, Anath Bandhu Das, Satya Narayan Jena, Sangram Sinha, In Vitro Clonal Propagation of Musa Sp. Cultivar Gopi: A Palatable Banana of Tripura, India, American Journal of Plant Biology. Vol. 3, No. 1, 2018, pp. 12-16. doi: 10.11648/j.ajpb.20180301.13
Copyright © 2018 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
INIBAP. International network for the improvement of banana and plantain. Annual Report,Montpellier, France.1998.
FAO.On-line database of the Food and Agricultural Organization of the United Nations.http://apps.fao.org/default.html.2001.
B. Aquil, T.J. Arif,N.B. Sarin,Q.M.R. Haq “Micro propagation and genetic transformation of banana for crop improvement and sustainable agriculture” J of Crop Sci, vol.3(2) pp. 64-77. 2012.
L. Tripathi “Genetic engineering for improvement of Musa protection in Africal” Afr J Biotechnol, vol. 2(12), pp. 503-508. 2003.
S.S. Cronauer, A.D. KriKorian “Multiplication of Musa from excised stem tips” Ann Bot (London),vol. 53, pp. 321-328. 1984.
R. DoreSwamy, L. Sahijram“Micropropagation of banana from male floral apices culture in vitro”Sci Hortic, vol. 40(3) pp.181-188.1989.
F.J. Novak, R. Afza, M. Van Duren, M. Perea-Dallos, B.V. Conger, T. Xiolang “Somatic embryogenesis and plant regeneration in suspension cultures of desert (AA, AAA) and cooking(AAB) bananas (Musa spp.)” Biotechnology, vol. 7, pp.154-159.1989.
F.X. Cote, R. Domergue, S. Monmarson, J. Schwendiman, C. Teisson, J.V. Escalant“Embryogenic cell suspension from the male flower of Musa AAA cv. Grand nain”Physiol Plant, vol. 97, pp. 285-290.1996.
M.A.K, Azad, M.N. Amin “Rapid clonal propagation of banana (Musa spp.) using in vitro culture of floral bud apex” Plant tissue cult, vol.1, pp. 1-9.2001.
L. Hrahsel, A. Basu, L. Sahoo “In vitro Propagation and assessment of the genetic fidelity of Musa acuminate (AAA) cv. Vaibalhla Derived from Immature male Flowers” Appl Biochem Biotechnol,vol. 172, pp.1530-1539.2014.
A. Rekha, S. C. Hiremath “Chromosome studies and Karyotype analysis of some triploid banana(Musa species) cultivars of AAA genomic group” J Hortl Sci, vol. 3(1), pp. 30-34.2008.
A.K. Sharma, A. Sharma. “Chromosome techniques: theory and practice”. Butterworths.London.1965.
N. Banerjee, A.K. Sharma “In vitro response as a reflection of genomic diversity in long-term cultures of Musa” Theoritical and Applied Genetics, vol. 76, pp. 733-736.1988.
U. Habiba, S. Reja, M. L. Saha, M. R. Khan, S. Hadiuzzaman“Endogenous bacterial contamination during in vitro culture of table banana: Identification and prevention” Plant Tissue Cult,vol.12,pp.117-124.2002.
S. Ahmed, A. Sharma, A. K. Singh, V. K. Wali, P. Kumari “In vitro multiplication of banana (Musasp.)cv. Grand Naine” Afr J Biotechnol, vol. 13(37), pp. 2696-2703.2014.
P. Karule, V. Dalvi, A. Kadu, R. Chaudhari, V. R. SubramaniamA. B. Patil “A commercial micropropagation protocol for virupakshi (AAB) banana via apical meristem” Afr J Biotechnol, vol.15(11), pp. 401-407. 2016.
K. Maharani, S. Beura, P.S. Munsi “A Fast Protocol for in vitro cloning of Banana (Musaacuminata) cv. Amritpani”Int J Curr Microbiol, App Sci, vol. 6(10), pp. 586-594.2017.
R. L. Jarret “In vitro propagation and genetic conservation of bananas and plaintains” In IBPGR Advising committee in vitro storage, report of the third meeting (appendix), IBPGR, Rome, Italy.1986.
D. Vuylsteke, Shoot tip culture for the propagation, conservation and exchange of Musa germ plasm.IBPGR. Rome. 1989.
W.C. Wong “In vitro propagation of banana (Musa spp.): Initiation, proliferation and development of shoot tip cultivars on define media” Plant cell Tissue and Organ Cult,vol. 6, pp.159-166.1986.
G. Arinative, P.R., Rubaihago, M. J. S. Magambo “Proliferation rate effects of cytokinins on banana(Musaspp) Cultivars”Sci Hortic, vol. 86, pp. 13-21.2000.
M. Sheidai, H. Aminpoor, Z. Noormohammadi, F. Farahani “Genetic variation induced by tissue culture in Banana (Musa acuminate L.) cultivar Cavandish Dwarf Masoud” Gene Conserve, vol. 9, pp. 1-10. 2009.
F. Farahani, H. Aminpoor, M. Sheidai, Z. Noormohammadi, M. H. Mazinan “An improved systemfor in vitro propagation of banana (Musa acuminate L.) cultivars” Asian J of Plant Sci, vol. 7, pp. 116-118. 2008.
J.N. Buah, E. Danso, K. J. Taah, E. A. Abole, E.A. Bediako,J. Asieidu, R. Baidoo “The effects of different concentrations of cytokinins on the in vitro multiplication of plaintain (Musa sp.)” Biotech,vol. 9(3), pp. 343-347. 2010.
N. Jafari, R.Y. Othman, N. Khalid “Effect of benzyl amino purine (BAP) pulsing on in vitro multiplication of Musa acuminata (banana)” Asian J of Plant Sci Res, vol. 10, pp. 2446-2454.2010.
J. Lohidas, D. Sujin “Effect of growth hormones in the micropropagation of Banana cv. Matti” Plant Archives, vol.15 (1), pp. 307-314. 2015.
Browse journals by subject