Volume 3, Issue 1, March 2018, Page: 12-16
In Vitro Clonal Propagation of Musa Sp. Cultivar Gopi: A Palatable Banana of Tripura, India
Rabindra Kumar Sinha, Cytogenetics and Plant Biotechnology Lab., Department of Botany, Tripura University, Suryamaninagar, India
Puja Rani Saha, Cytogenetics and Plant Biotechnology Lab., Department of Botany, Tripura University, Suryamaninagar, India
Anath Bandhu Das, Department of Botany, Utkal University, Bhubaneswar, India
Satya Narayan Jena, Genetics and Molecular Biology, Council of Scientific and Industrial Research-National Botanical Research Institute, Lucknow, India
Sangram Sinha, Cytogenetics and Plant Biotechnology Lab., Department of Botany, Tripura University, Suryamaninagar, India
Received: Apr. 18, 2018;       Accepted: May 3, 2018;       Published: May 30, 2018
DOI: 10.11648/j.ajpb.20180301.13      View  831      Downloads  64
Abstract
The present investigation has been concentrated on the establishment of an efficacious method for in vitro regeneration of a Musa cultivar Gopi of Tripura. Focus has been greatly anchored to the sterilization procedure for assuring successful aseptic culture establishment of this cultivar. The culture protocol for shoot induction and proliferation comprised of four different treatments in basal MS medium fortified with 6-Benzylaminopurine of varying concentrations. The optimum response was encountered in the treatment of BAP in MS medium at concentration of 8mg l-1 for shoot bud induction and multiplication. Subsequent shoot growth and multiplication was achieved through repeated subcultures in media containing lower concentration (4 mg l-1). Root initiation was stimulated and brought about by treatments with two different concentrations of Indole butyric acid. The best rooting was manifested in treatment with IBA at concentration of 2mg l-1. The present study is an approach towards successful establishment of a simple and rapid clonal propagation of a potent Musa cultivar Gopi having traditional and commercial value from this region of Tripura, North-east India.
Keywords
In Vitro Regeneration, Musa Cultivar Gopi, Benzylaminopurine, Indole Butyric Acid, Clonal Propagation, Tripura
To cite this article
Rabindra Kumar Sinha, Puja Rani Saha, Anath Bandhu Das, Satya Narayan Jena, Sangram Sinha, In Vitro Clonal Propagation of Musa Sp. Cultivar Gopi: A Palatable Banana of Tripura, India, American Journal of Plant Biology. Vol. 3, No. 1, 2018, pp. 12-16. doi: 10.11648/j.ajpb.20180301.13
Copyright
Copyright © 2018 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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