Volume 2, Issue 4, November 2017, Page: 142-147
Growth, Carotenoid Production, Antioxidant Capacity and Lipid Accumulation of Haematococcus sp. Under Different Light Intensities
Trung Vo, Department of Biochemistry and Toxicology, Nguyen Tat Thanh University, Ho Chi Minh, Viet Nam
Son Tran, Department of Biochemistry and Toxicology, Nguyen Tat Thanh University, Ho Chi Minh, Viet Nam
Phuc Nguyen, Department of Biochemistry and Toxicology, Nguyen Tat Thanh University, Ho Chi Minh, Viet Nam
Truc Mai, Department of Plant and Environmental Sciences, New Mexico State University, New Mexico, USA
Received: Oct. 12, 2017;       Accepted: Nov. 7, 2017;       Published: Dec. 20, 2017
DOI: 10.11648/j.ajpb.20170204.15      View  2117      Downloads  168
Haematococcus is a genus of unicellular green microalgae and it is known as an important resource of keto-carotenoid: Astaxanthin. In this work, a strain of Haematococcus sp. grew rapidly when cultivated in BBM medium under different light intensities from 30 to 120 μmol photons.m-2.s-1. Production of antioxidant compounds (carotenoid and phenolic compounds) as well as total antioxidant capacity and lipid accumulation of Haematococcus sp. increased with increase in light intensity especially after 21 days of cultivation. The results demonstrated that there was significant relation between production of carotenoid, phenolic compound and lipid accumulation in Haematococcus microalgae under high light cultural conditions.
Haematococcus, Carotenoid, Astaxanthin, Antioxidant Capacity
To cite this article
Trung Vo, Son Tran, Phuc Nguyen, Truc Mai, Growth, Carotenoid Production, Antioxidant Capacity and Lipid Accumulation of Haematococcus sp. Under Different Light Intensities, American Journal of Plant Biology. Vol. 2, No. 4, 2017, pp. 142-147. doi: 10.11648/j.ajpb.20170204.15
Copyright © 2017 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
A. Prieto, J. P. Canavate, M. García-González, “Assessment of carotenoid production by Dunaliella salina in different culture systems and operation regimes,” J. of Biotech., Vol. 151, pp. 180–185, 2011.
A. Shaish, A. Ben-Amotz, M. Avron, “Biosynthesis of β-carotene in Dunaliella,” Methods Enzymol., Vol. 213, pp. 439–444, 1992.
A. R. Domınguez-Bocanegra, I. G. Legarreta, F. M. Jeronimo, A. T. Campocosio, “Influence of environmental and nutritional factors in the production of astaxanthin from Haematococcus pluvialis,” Bioresource Technology, Vol. 92, pp. 209–214, 2004.
B. Duval, K. Shetty, W. H. Thomas, “Phenolic compounds and antioxidant properties in the snow alga Chlamydomonas nivalis after exposure to UV light,” J. Appl. Phycol., Vol. 11, pp. 559–566, 2000.
C. Faraloni and G. Torzillo, Chap. 9: Synthesis of Antioxidant Carotenoids in Microalgae in Response to Physiological Stress. In: D. J. Cvetkovic and G. S. Nikolic, Carotenoids, Intech, 2017, pp. 143-157.
C. Liang, Y. Zhai, D. Xu, N. Ye, X. Zhang, Y. Wang, W. Zhang and J. Yu, “Correlation between lipid and carotenoid synthesis and photosynthetic capacity in Haematococcus pluvialis grown under high light and nitrogen deprivation stress,” Grasas Aceites, Vol. 66 (2), 2015: e077. doi: http://dx.doi.org/10.3989/gya.0708142.
D. Tran, N. Doan, C. Louime, M. Giordano, S. Portilla, “Growth, antioxidant capacity and total carotene of Dunaliella salina DCCBC15 in a low cost enriched natural seawater medium,” World J. Microbiol Biotechnol., Vol. 30, pp. 317-322, 2014.
F. M. I. Natrah, F. M. Yusoff, M. Shariff, F. Abas and N. S. Mariana, “Screening of Malaysian indigenous microalgae for antioxidant properties and nutritional value,” J. Applied Phycol., Vol. 19, pp. 711-718, 2007.
G. Torzillo, T. Göksan, O. Isik and Ş. Gökpinar, “Photon irradiance required to support optimal growth and interrelations between irradiance and pigment composition in the green alga Haematococcus pluvialis,” Eur. J. Phycol., Vol. 40 (2), pp. 233–240, 2005.
H. C. Bold, “The morphology of Chlamydomonas chlamydogama sp. nov,” Bull. Torrey Bot. Club., Vol. 76, pp. 101–108, 1949.
H. Eom, C. G. Lee and E. Jin, “Gene expression profile analysis in astaxanthin-induced Haematococcus pluvialis using a cDNA microarray,” Planta, Vol. 223, pp. 1231–1242, 2006.
H. W. Bischoff, and H. C. Bold, Phycological Studies IV. Some Soil Algae From Enchanted Rock and Related Algal Species, University of Texas, Austin, 1963, pp. 1–95.
I. Hamed, F. Ozogul, Y. Özogul, and J. M. Regenstein, “Marine Bioactive Compounds and Their Health Benefits: A Review,” Comprehensive Reviews in Food Science and Food Safety, Vol. 14, pp. 446-645, 2015.
J. Eonseon, C-G. Lee and J. E. W. Polle, “Secondary Carotenoid Accumulation in Haematococcus (Chlorophyceae): Biosynthesis, Regulation, and Biotechnology,” J. Microbiol. Biotechnol., Vol. 16 (6), pp. 821–831, 2006.
J. Fábregas, A. Domínguez, M. Regueiro, A. Maseda, A. Otero, “Optimization of culture medium for the continuous cultivation of the microalga Haematococcus pluvialis,” Applied Microbiology and Biotechnology, Vol. 53 (5), pp. 530–535, 2002.
J. Kovácik, B. Klejdus, M. Backor, “Physiological responses of Scenedesmus quadricauda (Chlorophyceae) to UV-A and UV-C light,” Photochem. Photobiol., Vol. 86, pp. 612–616, 2010.
K. Goiris, K. Muylaert, I. Fraeye, I. Foubert, J. D. Brabanter and L. D. Cooman, “Antioxidant potential of microalgae in relation to their phenolic and carotenoid content,” J. Appl. Phycol., Vol. 24 (6), pp. 1477–1486, 2012.
K. Grünewald, J. Hirschberg and C. Hagen, “Ketocarotenoid Biosynthesis Outside of Plastids in the Unicellular Green Alga Haematococcus pluvialis,” The Journal of Biological Chemistry, Vol. 276 (8), pp. 6023–6029, 2001.
M. Hajimahmoodi, M. A. Faramarzi, N. Mohammadi, N. Soltani, M. R. Oveisi, N. Varcheh, “Evaluation of antioxidant properties and total phenolic contents of some strains of microalgae,” J. Appl. Phycol., Vol. 22, pp. 43–50, 2010.
M. Kobayashi, T. Kakizono, N. Nishio, S. Nagai, Y. Kurimura and Y. Tsuji, “Antioxidant role of astaxanthin in the green alga Haematococcus pluvialis.” Appl. Microbiol. Biotechnol., Vol. 48, pp. 351–356, 1997.
M. M. R. Shah, Y. Liang, J. J. Cheng and M. Daroch, “Astaxanthin-Producing Green Microalga Haematococcus pluvialis: From Single Cell to High Value Commercial Products,” Frontiers in Plant Science, Vol. 7, 1-28, 2016.
M. Zhekisheva, S. Boussiba, I. Khozin-Goldberg, A. Zarka, Z. Cohen, Accumulation of oleic acid in Haematococcus pluvialis (Chlorophyceae) under nitrogen starvation or high light is correlated with that of astaxanthin esters,” J. phycol., Vol. 38, 325-331, 2002.
M. A. Borowitzka, J. M. Huisman, A. Osborn, “Culture of the astaxanthin-producing green alga Haematococcus pluvialis. 1. Effect of nutrients on growth and cell type,” J Appl Phycol., Vol. 3, pp. 295–304, 1991.
P. Z. Margalith, “Production of ketocarotenoids by microalgae,” Appl. Microbiol. Biotechnol., Vol. 51, pp. 431–438, 1999.
R. Boateng, K. Faez-Sorkhabi, V. Veluvolu, P. Wu, “Extraction of Astaxanthin from Haematococcus pluvialis,” The Canadian Society for Bioengineering, pp. 1-16, 2016.
R. R. Ambati, S. M. Phang, S. Ravi and R. G. Aswathanarayana, “Astaxanthin: sources, extraction, stability, biological activities and its commercial applications—a review,” Mar. Drugs, Vol. 12, pp. 128–152, 2014.
R. Vidhyavathi, L. Venkatachalam, R. Sarada and G. A. Ravishankar, “Regulation of carotenoid biosynthetic genes expression and carotenoid accumulation in the green alga Haematococcus pluvialis under nutrient stress conditions,” Journal of Experimental Botany, Vol. 59 (6), pp. 1409–1418, 2008.
S. Albayrak, A. Aksoy, O. Sagdic, E. Hamzaoglu, “Compositions, antioxidant and antimicrobial activities of Helichrysum (Asteraceae) species collected from Turkey,” Food Chemistry, Vol. 119, pp. 114–122, 2010.
S. Boussiba and A. Vonshak, “Astaxanthin Accumulation in the Green Alga Haematococcus pluvialis,” Plant Cell Physiol., Vol. 32 (7), pp. 1077-1082, 1991.
S. Boussiba, “Carotenogenesis in the green alga Haematococcus pluvialis: Cellular physiology and stress response,” Physiologia Plantarum, Vol. 108, pp. 111–117, 2000.
S. K. Mishra, W. I. Suh, W. Farooq, M. Moon, A. Shrivastav, M. S. Park, J. W. Yang, “Rapid quantification of microalgal lipids in aqueous medium by a simple colorimetric method”, Bioresource Technology, Vol. 155, pp. 330-333, 2014.
S. N. Lim, P. C. K. Cheung, V. E. C. Ooi, P. O, Ang, “Evaluation of antioxidative activity of extracts from a brown seaweed, Sargassum siliquastrum,” J. Agric. Food Chem., Vol. 50: 3862–3866, 2002.
T. Göksan, İ. Ak, C. Kılıç, “Growth Characteristics of the Alga Haematococcus pluvialis Flotow as Affected by Nitrogen Source, Vitamin, Light and Aeration,” Turkish Journal of Fisheries and Aquatic Sciences, Vol. 11, pp. 377-383, 2011.
T. Yaltirak, B. Aslim, S. Ozturk, H. Alli, “Antimicrobial and antioxidant activities of Russula delica Fr.,” Food and Chemical Toxicology, Vol. 47, pp. 2052–2056, 2009.
U. Tripathi, R. Sarada and G. A. Ravishankar, “Effect of culture conditions on growth of green alga – Haematococcus pluvialis and astaxanthin production,” Acta Physiologiae Plantarum, Vol. 24 (3), pp. 23-329, 2002.
Y. Yang, J. M. Seo, A. Nguyen, T. X. Pham, H. J. Park, Y. Park, B. Kim, R. S. Bruno, and J. Lee, “Astaxanthin-Rich Extract from the Green Alga Haematococcus pluvialis Lowers Plasma Lipid Concentrations and Enhances Antioxidant Defense in Apolipoprotein E Knockout Mice,” The Journal of Nutrition, pp. 1-7, 2011.
Z. Gao, C. Meng, X. Zhang, D. Xu, X. Miao, Y. Wang, “Induction of salicylic acid (SA) on transcriptional expression of eight carotenoid genes and astaxanthin accumulation in Haematococcus pluvialis,” Enzyme Microb. Technol., Vol. 51, pp. 225–230, 2012.
Browse journals by subject