Detection of Bacteria Contaminating Milk and Milk Products in Tanzania by Conventional and Rapid Methods

Irene Mfilinge; Elisa Mwega; Lucy Chove; George Msalya

doi:doi:10.11648/j.ijmb.20230804.13

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Detection of Bacteria Contaminating Milk and Milk Products in Tanzania by Conventional and Rapid Methods

Irene Mfilinge^*, Elisa Mwega, Lucy Chove, George Msalya

Published in International Journal of Microbiology and Biotechnology (Volume 8, Issue 4)

Received: 4 October 2023 Accepted: 2 November 2023 Published: 17 November 2023

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Abstract

The aim of the present study was to evaluate the performance of rapid methods for identifying contaminating bacteria in milk and milk products in Tanzania. A total of 60 milk and milk product samples were collected along the dairy value chain in Morogoro Municipality. The samples included raw and boiled milk, local fermented milk, and industrial processed milk products. Laboratory analyses were conducted using the conventional tests (culture and confirmatory tests) as well as rapid methods (3M petrifilm plate and polymerase chain reaction (PCR). Total bacteria count and identification of Escherichia coli as well as Staphylococcus aureus were performed. Results had indicated that there is positive correlation (R=1) between the 3M petrifilm and conventional methods. On the other hand, the correlation between PCR and the conventional method was poor (R=-0.078 and -0.15 for E. coli and S. aureus identification respectively). Furthermore, the PCR identified more positive samples (8 for E. coli and 12 for S. aureus) compared to the conventional method (2 for E. coli and 5 for S. aureus). No significant difference (p˂0.05) was observed between 3M petrifilm and the conventional methods in tested boiled milk, local fermented milk, and industrial processed milk products; however, significant differences (p˃0.05) were observed in raw milk by both methods. Based on these results and the time to obtain them, it is confirmed that rapid methods performed better than the conventional methods for determining contamination and identifying bacteria in milk and milk products and are hence recommended for routine laboratory analysis in Tanzania.

Published in	International Journal of Microbiology and Biotechnology (Volume 8, Issue 4)
DOI	10.11648/j.ijmb.20230804.13
Page(s)	87-93
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2023. Published by Science Publishing Group

Keywords

Dairy Value Chain, 3M Petrifilms, Polymerase Chain Reaction (PCR), Escherichia coli, Staphylococcus aureus

References

[1]	International Livestock Research Institute (ILRI), (2011). Dairy value chain in Tanzania. Background proposal for CGIAR Research Program on Livestock and Fish. [http://cgspace.cgiar.org/bitstream/handle/10568/16962/LivestockFishDairyVCTanzania.pdf/sequence] site visited on 15/05/2021.
[2]	International Dairy Federation (IDF) (2013). The Economic Importance of Dairying IDF Factsheet – February 2013 [https://www.fil-idf.org›uploads›2016/04›the...PDF] site visited on 12/05/2021.
[3]	Food and Agriculture Organization (FAO), (2013). Milk and Dairy Products in Human Nutrition, edited by E. Muehlhoff, A. Bennett, and D. McMahon, United Nations, Rome, Italy. pp. 1-9.
[4]	Tschirley D., Reardon T., Dolislager M., Snyder J. Working Paper. 119. Helsinki, Finland: World Institute for Development Economics Research (WIDER); 2014. The rise of a middle class in East and Southern Africa: implications for food system transformation.
[5]	More, S. (2009). Global trends in milk quality: implications for the irish dairy industry. Irish Veterinary Journal, 62 (Suppl 4), S5.
[6]	Berhe, G., Wasihun, A. G., Kassaye, E., and Gebreselasie, K. (2020). Milk-borne bacterial health hazards in milk produced for commercial purpose in Tigray, northern Ethiopia. BMC Public Health 20 (1).
[7]	Bertu, W. J., Dapar, M., Gusi, A. M., Ngulukun, S. S., Leo, S., and Jwander, L. D. (2010). Prevalence of brucella antibodies in marketed milk in Jos and environs. African Journal of Food Science 4 (2): 062 – 064.
[8]	Msalya, G. (2017). Contamination Levels and Identification of Bacteria in Milk Sampled from Three Regions of Tanzania: Evidence from Literature and Laboratory Analyses. Veterinary Medicine International 2017: 1–10.
[9]	Ngasala, J. U. B., Nonga, H. E., and Mtambo, M. M. A. (2015). Assessment of raw milk quality and stakeholders’ awareness on milk-borne health risks in Arusha City and Meru District, Tanzania. Tropical Animal Health and Production 47 (5): 927–932.
[10]	Mdegela, R. H., Ryoba, R., and Karimuribo, E. D. (2009). Prevalence of clinical and subclinical mastitis and quality of milk on smallholder dairy farms in Tanzania. Tydskr South African Veterinary Association 80 (3): 163–168.
[11]	EAC (East African Community), 2006 “Raw Cow Milk Specification in East African Community Standard. pp. 1-2.
[12]	Tanzania Dairy Board (TDB) (2019). State of Dairy Industry of Tanzania 2018/19.
[13]	Souza, L. M. J. de, Costa, A. C., Nero, L. A., Couto, E. P., and Ferreira, M. de A. (2015). Evaluation of Petrifilm™ system compared with traditional methodology in count of indicators of sanitary-hygienic quality and pathogenic microorganisms in sheep milk. Food Science and Technology (Campinas) 35 (2): 375–379.
[14]	Mandal, P. K., Biswas, A. K., Choi, K. and Pal, U. K. (2011). Methods for rapid detection of foodborne pathogens: an overview. American Journal of Food Technology 6: 87–102.
[15]	Gunasekera, T. S., Attfield, P. V. and Veal, D. A. (2000). A Flow Cytometry Method for Rapid Detection and Enumeration of Total Bacteria in Milk. Applied and Environmental Microbiology 66 (3): 1228–1232.
[16]	Losito, F., Arienzo, A., Bottini, G., Priolisi, F. R., Mari, A., Antonini, G. (2014). Microbiological safety and quality of Mozzarella cheese assessed by the microbiological survey method. Journal of Dairy Science 97: 46–55.
[17]	Antonini, G., Arienzo, A., Losito, F. and Stalio, O. (2016). Comparison of Uncertainty between Traditional and Alternative Methods for Food Microbiological Analysis. American Journal of Food Technology 11: 29–36.
[18]	ISO (2013). Microbiology of the food chain – Horizontal method for the enumeration of microorganisms. International Organization for Standardization (ISO 4833-1: 2013). pp. 1-10.
[19]	ISO (2007). Microbiology of food and animal feeding stuffs- general requirements and guidance for microbiological examination. International Standards Organization (ISO 7218-2007). pp. 8–15.
[20]	ISO (1999). Microbiology of food and animal feeding stuffs – Horizontal method for enumeration of coagulase-positive staphylococci (Staphylococcus aureus and other species). International Organization for Standardization (ISO 6888-1:1999). pp. 1-14.
[21]	ISO (2004). Microbiology of food and animal feeding stuffs – Horizontal method for the detection and enumeration of Enterobacteriaceae. International Organization for Standardization (ISO 21528-2:2004). pp. 1-12.
[22]	Onohuean, H., & Igere, B. E. (2022). Occurrence, Antibiotic Susceptibility and Genes Encoding Antibacterial Resistance of Salmonella spp. and Escherichia coli From Milk and Meat Sold in Markets of Bushenyi District, Uganda. Microbiology insights, 15, 11786361221088992.
[23]	McClure, J.-A., Conly, J. M., Lau, V., Elsayed, S., Louie, T., Hutchins, W., & Zhang, K. (2006). Novel Multiplex PCR Assay for Detection of the Staphylococcal Virulence Marker Panton-Valentine Leukocidin Genes and Simultaneous Discrimination of Methicillin-Susceptible from -Resistant Staphylococci. Journal of Clinical Microbiology, 44 (3), 1141–1144.
[24]	Scott, M., Flaherty, D., & Currall, J. (2013). Statistics: are we related? Journal of Small Animal Practice, 54 (3), 124–128.
[25]	Alhelfi, N. A. (2016). Assessment of the Efficiency of Petrifim Method in Study of Bacteriological Quality of Some Homemade Dairy Products in Local Market of Basra. Food Science and Quality Management 54: 39-46.
[26]	Park, J., and Kim, M. (2013). Comparison of Dry Medium Culture Plates for Mesophilic Aerobic Bacteria in Milk, Ice Cream, Ham, and Codfish Fillet Products. Prev Nutr Food Sci. 18 (4): 269–272.
[27]	Lakmini, N. K. A., and Madhujith, T. (2012). Comparison of Performance of Rapid Petrifilm Test Method and Standard Test Method for Enumeration of Aerobic Microorganisms, Coliforms and E. coli in Food. Tropical Agricultural Research 23 (4): 363 – 369.
[28]	de Sousa, G. B., Tamagnini, L. M., González, R. D., and Budde, C. E. (2005). Evaluation of Petrifilm method for enumerating aerobic bacteria in Crottin goat cheese. Rev. Argent Microbiology 37 (4): 214-226.
[29]	Cho, M. H., Bae, E. K., Ha, S. D., Park, Y. S., Mok, C. K., Hong, K. P., Kim, S. P., and Park, J. Y. (2005). Evaluation of dry rehydratable film method for enumeration of microorganisms in meat, dairy and fishery products. Korean Journal of Food Science and Technology 37: 294–300.
[30]	Khudhir, Z. S., Najim, N. H., and Yousif, D. H. (2013). comparison between the petrifilm™ and the conventional methods for enumerating aerobic bacteria and E. coli in locally produced soft cheese in baghdad Bas. Journal of Veterinary Research 12 (2).
[31]	Rosmini, M. R., Signorini, M. L., Schneider, R., and Bonazza, J. C. (2004). Evaluation of two alternative techniques for counting mesophilic aerobic bacteria in raw milk. Food Control 15 (1): 39-44.
[32]	Freitas, R., Nero, L. A., and Carvalho, A. F. (2009). Technical note: enumeration of mesophilic aerobes in milk: evaluation of standard official protocols and Petrifilm aerobic count plates. Journal of Dairy Science 92 (7): 3069-3073.
[33]	Carvalho, C. M., Oliveira, A. J. G., and Rosa, C. (2002). The Petrifilm system as an alternative for traditional methods for total count of aerobic microorganisms and total coliform in refrigerated raw milk. Higiene Alimentar Magazine 16 (100): 116-126.
[34]	Rezaei, M., Khomeiri, M., Ebrahimi, M., Kiani, S., and Raeisi, M. (2019). Biochemical and Molecular Identification of Listeria monocytogenes and Escherichia coli in the Raw Milk Samples Delivered to the Dairy Farms in Golestan Province, Iran. J. Hum. Environ. Health Promot 5 (4): 160-164.
[35]	Federica, G., Paolo, B., Andrea, S., Angelo, P., Simonetta, A., Norma, A., and Bolzoni, G. (2013). Four-year monitoring of foodborne pathogens inraw Milk sold. Journal of Food Protection 76 (11): 1902–1907.
[36]	Kawasaki, S., Fratamico, P. M., Horikoshi, N., Okada, Y., Takeshita, K., Sameshima, T., and Kawamoto, S. (2009). Evaluation of a Multiplex PCR System for Simultaneous Detection of Salmonella spp., Listeria monocytogenes, and Escherichia coli O157:H7 in Foods and in Food Subjected to Freezing. Foodborne Pathogens and Disease 6 (1): 81–89.
[37]	Bastam, M. M., Jalili, M., Pakzad, I., Maleki, A., and Ghafourian, S. (2021). Pathogenic bacteria in cheese, raw and pasteurized milk. Veterinary Medicine and Science 2021: 1–5.
[38]	Bharathy, S., Gunaseelan, L., Porteen, K., and Bojira, M. (2015). Prevalence of staphylcoccus aureus in raw milk: can it be a potential public health threat? International Journal of Advanced Research 3 (2): 801-806.
[39]	Ahmadi, M., Rohani, S. M. R., and Ayremlou, N. (2010). Detection of Staphylococcus aureus inmilk by PCR. Comp Clin Pathol 19: 91–94.
[40]	Yang, Y., Su, X., Yuan, Y., Kang, C., Li, Y., Zhang, Wei, and Zhong, X. (2007). Detection of Staphylococcus aureus in Dairy Products by Polymerase Chain Reaction Assay. Agricultural Sciences in China 6 (7): 857–86.
[41]	Makino, H., Fujimoto, J., and Watanabe, K. (2010). Development and evalua-tion of a real time quantitative PCR assay for detection and enumera-tion of yeasts of public health interest in dairy products. International Journal of Food Microbiology 140: 76–83.
[42]	Farhoudi, A., Ghajarbeygi, P., Jozani, R. A. J., Mahmoudi, R., and Mardani, K. (2019). Comparison of real-time PCR and cultural method for detection of bacterial load in pasteurizedmilk. J. Food Saf. 2019: e12624.
[43]	Steele, N. M., Williamson, J. H., Thresher, R., Laven, R. A., and Hillerton, J. E. (2017). Evaluating a commercial PCR assay against bacterial culture for diagnosing Streptococcus uberis and Staphylococcus aureus throughout lactation. Journal of Dairy Science 100 (5): 3816–3824.

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APA Style

Mfilinge, I., Mwega, E., Chove, L., Msalya, G. (2023). Detection of Bacteria Contaminating Milk and Milk Products in Tanzania by Conventional and Rapid Methods. International Journal of Microbiology and Biotechnology, 8(4), 87-93. https://doi.org/10.11648/j.ijmb.20230804.13

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ACS Style

Mfilinge, I.; Mwega, E.; Chove, L.; Msalya, G. Detection of Bacteria Contaminating Milk and Milk Products in Tanzania by Conventional and Rapid Methods. Int. J. Microbiol. Biotechnol. 2023, 8(4), 87-93. doi: 10.11648/j.ijmb.20230804.13

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Mfilinge I, Mwega E, Chove L, Msalya G. Detection of Bacteria Contaminating Milk and Milk Products in Tanzania by Conventional and Rapid Methods. Int J Microbiol Biotechnol. 2023;8(4):87-93. doi: 10.11648/j.ijmb.20230804.13

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@article{10.11648/j.ijmb.20230804.13,
  author = {Irene Mfilinge and Elisa Mwega and Lucy Chove and George Msalya},
  title = {Detection of Bacteria Contaminating Milk and Milk Products in Tanzania by Conventional and Rapid Methods},
  journal = {International Journal of Microbiology and Biotechnology},
  volume = {8},
  number = {4},
  pages = {87-93},
  doi = {10.11648/j.ijmb.20230804.13},
  url = {https://doi.org/10.11648/j.ijmb.20230804.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijmb.20230804.13},
  abstract = {The aim of the present study was to evaluate the performance of rapid methods for identifying contaminating bacteria in milk and milk products in Tanzania. A total of 60 milk and milk product samples were collected along the dairy value chain in Morogoro Municipality. The samples included raw and boiled milk, local fermented milk, and industrial processed milk products. Laboratory analyses were conducted using the conventional tests (culture and confirmatory tests) as well as rapid methods (3M petrifilm plate and polymerase chain reaction (PCR). Total bacteria count and identification of Escherichia coli as well as Staphylococcus aureus were performed. Results had indicated that there is positive correlation (R=1) between the 3M petrifilm and conventional methods. On the other hand, the correlation between PCR and the conventional method was poor (R=-0.078 and -0.15 for E. coli and S. aureus identification respectively). Furthermore, the PCR identified more positive samples (8 for E. coli and 12 for S. aureus) compared to the conventional method (2 for E. coli and 5 for S. aureus). No significant difference (p˂0.05) was observed between 3M petrifilm and the conventional methods in tested boiled milk, local fermented milk, and industrial processed milk products; however, significant differences (p˃0.05) were observed in raw milk by both methods. Based on these results and the time to obtain them, it is confirmed that rapid methods performed better than the conventional methods for determining contamination and identifying bacteria in milk and milk products and are hence recommended for routine laboratory analysis in Tanzania.
},
 year = {2023}
}

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TY - JOUR
T1 - Detection of Bacteria Contaminating Milk and Milk Products in Tanzania by Conventional and Rapid Methods
AU - Irene Mfilinge
AU - Elisa Mwega
AU - Lucy Chove
AU - George Msalya
Y1 - 2023/11/17
PY - 2023
N1 - https://doi.org/10.11648/j.ijmb.20230804.13
DO - 10.11648/j.ijmb.20230804.13
T2 - International Journal of Microbiology and Biotechnology
JF - International Journal of Microbiology and Biotechnology
JO - International Journal of Microbiology and Biotechnology
SP - 87
EP - 93
PB - Science Publishing Group
SN - 2578-9686
UR - https://doi.org/10.11648/j.ijmb.20230804.13
AB - The aim of the present study was to evaluate the performance of rapid methods for identifying contaminating bacteria in milk and milk products in Tanzania. A total of 60 milk and milk product samples were collected along the dairy value chain in Morogoro Municipality. The samples included raw and boiled milk, local fermented milk, and industrial processed milk products. Laboratory analyses were conducted using the conventional tests (culture and confirmatory tests) as well as rapid methods (3M petrifilm plate and polymerase chain reaction (PCR). Total bacteria count and identification of Escherichia coli as well as Staphylococcus aureus were performed. Results had indicated that there is positive correlation (R=1) between the 3M petrifilm and conventional methods. On the other hand, the correlation between PCR and the conventional method was poor (R=-0.078 and -0.15 for E. coli and S. aureus identification respectively). Furthermore, the PCR identified more positive samples (8 for E. coli and 12 for S. aureus) compared to the conventional method (2 for E. coli and 5 for S. aureus). No significant difference (p˂0.05) was observed between 3M petrifilm and the conventional methods in tested boiled milk, local fermented milk, and industrial processed milk products; however, significant differences (p˃0.05) were observed in raw milk by both methods. Based on these results and the time to obtain them, it is confirmed that rapid methods performed better than the conventional methods for determining contamination and identifying bacteria in milk and milk products and are hence recommended for routine laboratory analysis in Tanzania.

VL - 8
IS - 4
ER -

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Author Information

Irene Mfilinge

Department of Animal, Aquaculture and Range Sciences (DAARS), Sokoine University of Agriculture (SUA), Morogoro, Tanzania

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Elisa Mwega

Department of Veterinary Microbiology, Parasitology and Biotechnology, Sokoine University of Agriculture (SUA), Morogoro, Tanzania
Lucy Chove

Department of Food Science and Agro-Processing Engineering, Sokoine University of Agriculture (SUA), Morogoro, Tanzania
George Msalya

Department of Animal, Aquaculture and Range Sciences (DAARS), Sokoine University of Agriculture (SUA), Morogoro, Tanzania; Tanzania Dairy Board (TDB), Dodoma, Tanzania

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APA Style

Mfilinge, I., Mwega, E., Chove, L., Msalya, G. (2023). Detection of Bacteria Contaminating Milk and Milk Products in Tanzania by Conventional and Rapid Methods. International Journal of Microbiology and Biotechnology, 8(4), 87-93. https://doi.org/10.11648/j.ijmb.20230804.13

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ACS Style

Mfilinge, I.; Mwega, E.; Chove, L.; Msalya, G. Detection of Bacteria Contaminating Milk and Milk Products in Tanzania by Conventional and Rapid Methods. Int. J. Microbiol. Biotechnol. 2023, 8(4), 87-93. doi: 10.11648/j.ijmb.20230804.13

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AMA Style

Mfilinge I, Mwega E, Chove L, Msalya G. Detection of Bacteria Contaminating Milk and Milk Products in Tanzania by Conventional and Rapid Methods. Int J Microbiol Biotechnol. 2023;8(4):87-93. doi: 10.11648/j.ijmb.20230804.13

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@article{10.11648/j.ijmb.20230804.13,
  author = {Irene Mfilinge and Elisa Mwega and Lucy Chove and George Msalya},
  title = {Detection of Bacteria Contaminating Milk and Milk Products in Tanzania by Conventional and Rapid Methods},
  journal = {International Journal of Microbiology and Biotechnology},
  volume = {8},
  number = {4},
  pages = {87-93},
  doi = {10.11648/j.ijmb.20230804.13},
  url = {https://doi.org/10.11648/j.ijmb.20230804.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijmb.20230804.13},
  abstract = {The aim of the present study was to evaluate the performance of rapid methods for identifying contaminating bacteria in milk and milk products in Tanzania. A total of 60 milk and milk product samples were collected along the dairy value chain in Morogoro Municipality. The samples included raw and boiled milk, local fermented milk, and industrial processed milk products. Laboratory analyses were conducted using the conventional tests (culture and confirmatory tests) as well as rapid methods (3M petrifilm plate and polymerase chain reaction (PCR). Total bacteria count and identification of Escherichia coli as well as Staphylococcus aureus were performed. Results had indicated that there is positive correlation (R=1) between the 3M petrifilm and conventional methods. On the other hand, the correlation between PCR and the conventional method was poor (R=-0.078 and -0.15 for E. coli and S. aureus identification respectively). Furthermore, the PCR identified more positive samples (8 for E. coli and 12 for S. aureus) compared to the conventional method (2 for E. coli and 5 for S. aureus). No significant difference (p˂0.05) was observed between 3M petrifilm and the conventional methods in tested boiled milk, local fermented milk, and industrial processed milk products; however, significant differences (p˃0.05) were observed in raw milk by both methods. Based on these results and the time to obtain them, it is confirmed that rapid methods performed better than the conventional methods for determining contamination and identifying bacteria in milk and milk products and are hence recommended for routine laboratory analysis in Tanzania.
},
 year = {2023}
}

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VL - 8
IS - 4
ER -

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